High Throughput Screening Review

I conduct CRISPR array screens to identify genes whose knock-out promote a specific endpoint of interest. My role consists of growing and splitting cells in culture, inducing Cas9 expression and gene editing through different methods, using high-throughput liquid handling systems to work with 384-wells plate for the screen, as well as electron microscopes for imaging as part of immunofluorescence assays. I also prepared write ups of my experiments for my supervisor.

After the first few months, I was basically given full autonomy over my work, with the opportunity to regularly ask my supervisor or other colleagues for guidance. However, I was regularly evaluated on my progress through reports and oral presentations to my supervisor and the wider team I belonged to. Moreover, the project I was assigned to was not high-priority.

I have developed basic skills in wet and dry lab work, such as cell culturing, immunofluorescence, various transfection techniques, gene KOs via a CRISPR-based system, Western Blotting, imaging, data analysis and report writing. I have also been exposed to a variety of projects inside the company from different departments, giving me insight into the nature of roles other than mine.

I was in regular contact with both my supervisor and line manager. The atmosphere was very relaxed and I felt comfortable asking questions to anyone within my team. I was given shadowing opportunities as introduction to new techniques, with me attempting them under supervision before continuing on my own as I gained technical confidence.

Relaxed and open minded, although the size of the team makes it hard to keep in contact with everyone without regularly and proactively reaching out.

The placement helped me get clearer ideas about what type of career I would like to pursue in the future.

Yes